PhD in Structural biochemistry of Host /Pathogen interaction, Glycobiology
Identification du poste :
Fonctions | Doctorant / PhD |
Catégorie | A |
Corps | Chercheur |
Quotité | 100 % |
- Assignment (place of work): Institute of Structural Biology (IBS – UMR 5075) – Grenoble, France
- Contract dates: from 1/10/2025 to 30/09/2028 (36 months)
Description de la structure
Located on the international EPN campus (ESRF/ILL/EMBL/IBS), the Institute of Structural Biology (IBS) is a national and international player in the field of integrated structural biology. At the same time a research centre, a technical platform, a reception and scientific training site, the IBS aims to develop research in structural biology, a field of research that is crucial for the understanding of fundamental biological mechanisms. It is based on 12 state-of-the-art platforms. As a joint research unit (CEA-CNRS-UGA) the IBS is composed of 19 research groups, each of these groups offering a multidisciplinary approach, at the frontiers of biology, physics and chemistry, in coherence with 3 research axes. Nearly 300 people work there, researchers, doctoral students, engineers and technicians in a multicultural and international environment. The recruited person will join the Membrane and Pathogens group.
Job duties and main activities :
Precise project name :
Investigating S. aureus Recognition by Host Receptor Proteins.
This position is one of 15 doctoral positions in a European doctoral network, called Aureus, whose members are spread across 9 European countries, and which will be operational from 2022 to 2029. The groups involved range from synthetic chemistry and immunology to structural, computational and cellular biology.
This particular PhD project aims to study in depth the recognition of S. aureus by specific receptor proteins, including C- type lectin receptors (CLRs) to obtain more in-depth information on the critical molecular motifs recognized that could be used in certain potential therapeutic strategies. The capacity of CLRs to recognize molecular motif from S. aureus has been already documented but only for a limited number of CLR and thus it is still a poorly understood aspect of host- pathogen interactions. We have developed expertise in the production of a library of CLRs and the characterization of their interaction with carbohydrate-based motifs from pathogens.
Main activities :
Thus, in this project, the recruited PhD will: 1) produce and engineer different proteins, including lectins at different oligomeric states ; 2) screen and identify the diversity of receptor proteins able to recognize selectively molecular component of S. aureus cell wall (in collaboration with other PhDs, DC1 and DC5 within the European network); 3) characterize proteins interaction with identified ligand investigating their binding affinity and kinetics through biophysical assays, such as ITC, SPR ( and/or BLI.
In collaboration with 3 others PhDs within the Aureus Network, the structural basis of receptor/ligand complexes will be elucidated. Cryo-electron microscopy for larger complexes will be considered. The fine characterization of binding
Priority skills expected :
- Professional skills/know-how : Prior experience in recombinant protein production, biochemistry, biophysics of interaction study (SPR, BLI or ITC) and/or background on structural biology.
- Personal skills : Organizational skills, fluency in English (level C1 spoken and written), ability to work independently and as a team player in a multidisciplinary environment. Good communication skills (in the context of a multi-partner project on a European scale).
Supervisory role (hierarchical or functional) : No
Desired professional experience : beginner
Training, diploma, experience required : Master degree in Biochemistry, Structural biology, Chemical biology or Pharmacy.
Gross salary: 2700 € month + 600 euros of mobility allowance
Contacts for questions :
- related to functions :
Dr. Franck Fieschi, Professor at Université Grenoble-Alpes, Franck.fieschi@ibs.fr
- about the position :
Valérie LANARI, HR Manager valerie.lanari@ibs.fr
Application to the position :Please follow this link :https://emploi.univ-grenoble-alpes.fr/job-offers/phd-in-structural-biochemistry-of-host-pathogen-interaction-aureus-marie-curie-doctoral-network-1568438.kjsp?RH=1135797159702996